PUBLICATIONS

JansenLab papers
Lars´ postdoc papers
Lars´ pre-PhD papers
Lars´ even earlier Papers

JansenLab papers


Pawel Mikulski, Sahar S.H. Tehrani, Anna Kogan, Izma Abdul-Zani, Emer Shell, Brent J. Ryan and Lars E.T. Jansen (2023) Transcriptional memory is conferred by combined heritable maintenance and local removal of selective chromatin modifications BioRxiv Preprint


 Sahar SH Tehrani, Anna Kogan, Pawel Mikulski and Lars ET Jansen (2023) Remembering foods and foes: emerging principles of transcriptional memory Nature, Cell Death & Differentiation


 Sahar SH Tehrani, Pawel Mikulski, Izma Abdul-Zani, João F Mata, Wojciech Siwek and Lars ET Jansen (2023) STAT1 is required to establish but not maintain IFNγ-induced transcriptional memory EMBO Journal, e112259

 Sebastiaan J.W. van den Berg and Lars E.T. Jansen (2023) SUMO control of centromere homeostasis Front. Cell Dev. Biol., doi.org/10.3389/fcell.2023.1193192





 Sebastiaan J.W. van den Berg, Samuel East, Sreyoshi Mitra and Lars E.T. Jansen (2023) p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A MBoC, doi.org/10.1091/mbc.E23-01-0035


 Dragan Stajic and Lars E. T. Jansen (2021) Empirical evidence for epigenetic inheritance driving evolutionary adaptation Phil. Trans. R. Soc., B3762020012120200121



 Marina Murillo-Pineda, Luis P. Valente, Marie Dumont, João F. Mata, Daniele Fachinetti and Lars E.T. Jansen (2021) Induction of spontaneous human neocentromere formation and long-term maturation. Journal of Cell Biology, 220 (3): e202007210
Highlighed by Ben Carty and Elaine Dunleavy

Wojciech Siwek, Sahar S.H. Tehrani, João F. Mata and Lars E.T. Jansen (2020) Activation of clustered IFNg target genes drives cohesin-controlled transcriptional memory. Molecular Cell, doi.org/10.1016/j.molcel.2020.10.005




 Inês Milagre, Carolina Pereira, Raquel Oliveira and Lars E.T. Jansen (2020) Reprogramming of Human Cells to Pluripotency Induces CENP-A Chromatin Depletion. Open Biology,10: 200227



 Sebastiaan J. W. van den Berg and Lars E.T. Jansen (2020) Centromeres: Genetic input to calibrate an epigenetic feedback loop EMBO Journal, e106638




 Sreyoshi Mitra, Bharath Srinivasan and Lars E.T. Jansen (2020) Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control Journal of Cell Biology, 219 (10): e202005099



 Georg O. M. Bobkov, Anming Huang, Sebastiaan J. W. van den Berg, Sreyoshi Mitra, Eduard Anselm1, Vasiliki Lazou1, Sarah Schunter, Regina Feederle, Axel Imhof, Alexandra Lusser, Lars E. T. Jansen and Patrick Heun (2020) Spt6 is a maintenance factor for centromeric CENP-A Nature Communications, 11: 2020

 Marina Murillo-Pineda and Lars E.T. Jansen (2020) Genetics, epigenetics and back again: Lessons learned from neocentromeres. Experimental Cell Research doi.org/10.1016/j.yexcr.2020.111909



 Sreyoshi Mitra, Dani L. Bodor, Ana F. David, Izma Abdul-Zani João F. Mata, Beate Neumann, Sabine Reither, Christian Tischer and Lars E.T. Jansen (2020) Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nature Communications, 11: 501

Dragan Stajic, Lília Perfeito and Lars E.T. Jansen (2019) Epigenetic gene silencing alters the mechanisms and rate of evolutionary adaptation. Nature Ecology & Evolution, 3: 491-8



 Wojciech Siwek, Mariluz Gómez-Rodríguez, Daniel Sobral, Ivan R. Corrêa Jr and Lars E.T. Jansen (2018) time-ChIP: A method to determine long-term locus-specific nucleosome inheritance. Methods Mol. Biol., 1832: 131-58


Ana Stankovic and Lars E.T Jansen (2017) Quantitative Microscopy Reveals Centromeric Chromatin Stability, Size, and Cell Cycle Mechanisms to Maintain Centromere Homeostasis. Prog Mol Subcell Biol., 56: 139-162


Ana Stankovic, Lucie Y. Guo, João F. Mata, Dani L. Bodor, Xing-Jun Cao, Aaron O. Bailey, Jeffrey Shabanowitz, Donald F. Hunt, Benjamin A. Garcia, Ben E. Black and Lars E.T Jansen (2017) A dual inhibitory mechanism sufficient to maintain cell cycle restricted CENP-A assembly. Molecular Cell, 65: 231–246

Aimee M Deaton, Mariluz Gomez-Rodriguez, Jakub Mieczkowski, Michael Y Tolstorukov, Sharmistha Kundu, Ruslan I Sadreyev, Lars E.T. Jansen and Robert E Kingston (2016) Enhancer regions show high histone H3.3 turnover that changes during differentiation eLife, 5:e15316

Samantha J Falk, Lucie Y Guo, Nikolina Sekulic, Evan M Smoak, Tomoyasu Mani, Glennis A Logsdon, Kushol Gupta, Lars E T Jansen, Gregory D Van Duyne, Sergei A Vinogradov, Michael A Lampson and Ben E Black (2015) CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere. Science, 348: 699-703

Dani L. Bodor, João F. Mata, Mikhail Sergeev, Ana Filipa David, Kevan J. Salimian, Tanya Panchenko, Don W. Cleveland, Ben E. Black, Jagesh V. Shah and Lars E.T. Jansen (2014) The quantitative architecture of centromeric chromatin. eLife, 2014;3:e02137


Daniele Fachinetti, H. Diego Folco, Yael Nechemia-Arbely, Luis P. Valente, Kristen Nguyen, Alex J. Wong, Quan Zhu, Andrew J. Holland, Arshad Desai, Lars E. T. Jansen and Don W. Cleveland (2013) A two-step mechanism for epigenetic specification of centromere identity and function. Nature Cell Biol., 15: 1056-66.

Dani L. Bodor and Lars E.T. Jansen (2013) How two become one: HJURP dimerization drives CENP-A assembly. EMBO J., 32: 2090-2.




William C. Earnshaw et al., (2013) Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant. Chromosome Res., 21: 101-6.

Dani L. Bodor, Luis P. Valente, João F. Mata, Ben E. Black and Lars E.T. Jansen (2013) Assembly in G1 phase and Long-Term Stability are Unique Intrinsic Features of CENP-A nucleosomes. Mol. Biol. Cell., 24: 923-932


Ana Stankovic and Lars E.T. Jansen (2013) Reductionism at the vertebrate kinetochore. Journal of Cell Biology, 200: 7-8





Mariluz Gómez-Rodríguez and Lars E.T. Jansen (2013) Basic properties of epigenetic systems: lessons from the centromere. Curr Opin Genet Dev., 23: 219-27


Luis P. Valente, Mariana C.C. Silva and Lars E.T. Jansen (2012)Temporal control of epigenetic centromere specification. Chromosome Research, 20:481-92




Dani L. Bodor, Mariluz Gómez Rodríguez, Nuno Moreno and Lars E.T. Jansen (2012) Analysis of protein turnover by quantitative SNAP-based pulse-chase imaging. Current Protocols in Cell Biology, Unit 8.8.1-8.8.34, June 2012

João F. Mata, Telma Lopes, Rui Gardner and Lars E.T. Jansen (2012) A rapid FACS-based strategy to isolate human gene knockin and knockout clones. PLoS ONE, 7:e32646



Lars E.T. Jansen (2012) Sowing the seeds of centromeres. Science, 335: 299-300





Mariana C.C. Silva, Dani L. Bodor, Madison E. Stellfox, Nuno M.C. Martins, Helfrid Hochegger, Daniel R. Foltz and Lars E.T. Jansen (2012) Cdk activity couples epigenetic centromere inheritance to cell cycle progression. Developmental Cell, 22: 52-63
(Highlighed by Kaitlin M. Stimpson and Beth A. Sullivan)

Dominique Ray Gallet, Adam Woolfe, Isabelle Vassias, Céline Pellentz, Nicolas Lacoste, Aastha Puri, David C. Schultz, Nikolay A. Pchelintsev, Peter D. Adams, Lars E.T. Jansen and Geneviève Almouzni (2011) Dynamics of histone H3 deposition in vivo reveal a gap filling mechanism for H3.3 to maintain chromatin integrity. Molecular Cell, 44: 928-41

Jan H. Bergmann, Mariluz Gómez Rodríguez, Nuno M. C. Martins, Hiroshi Kimura, David A. Kelly, Hiroshi Masumoto, Vladimir Larionov, Lars E. T. Jansen and William C Earnshaw (2011) Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore. EMBO Journal, 30:328-40

Ben E. Black, Lars E.T. Jansen, Daniel R. Foltz and Don W. Cleveland (2010) Centromere Identity, Function, and Epigenetic Propagation across Cell Divisions. Cold Spring Harb Symp Quant Biol, 75:403-18


Mariana C.C. Silva and Lars E.T. Jansen (2009) At the right place at the right time: Novel CENP-A binding proteins shed light on centromere assembly. Chromosoma, 118: 567-74



Christopher W. Carroll, Mariana C.C. Silva, Kristina M. Godek, Lars E.T. Jansen and Aaron F. Straight (2009) Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N. Nature Cell Biol., 11, 896-902


Lars´ Postdoc Papers

Ludwig Institute for Cancer Research, La Jolla, CA


Alexander Samoshkin, Alexei Arnaoutov, Lars E. T. Jansen, Ilia Ouspenski, Louis Dye, Tatiana Karpova, James McNally, Mary Dasso, Don W. Cleveland and Alexander Strunnikov (2009). Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation. PLoS One, 4:e6831.

Daniel R. Foltz, Lars E.T. Jansen, Aaron O. Bailey, John R. Yates III, Emily A. Bassett, Stacey Wood, Ben E. Black and Don W. Cleveland (2009) Centromere-Specific Assembly of CENP-A Nucleosomes Is Mediated by HJURP. Cell, 137, 472-84


Lars E.T. Jansen, Ben E. Black, Daniel R. Foltz, and Don W. Cleveland (2007) Propagation of centromeric chromatin requires exit from mitosis. J Cell Biol., 176, 795-805
Highlighed by Christopher W. Carroll and Aaron F. Straight and Mitch Leslie.


Ben E. Black*, Lars E.T. Jansen*, Paul S. Maddox, Daniel R. Foltz, Arshad B. Desai, Jagesh V. Shah and Don W. Cleveland (2007) Centromere Identity Maintained by Nucleosomes Assembled with Histone H3 Containing the CENP-A Targeting Domain. Molecular Cell, 25, 309-22 *equal contributors

Daniel R. Foltz, Lars E.T. Jansen, Ben E. Black, Aaron O. Bailey, John R. Yates III and Don W. Cleveland (2006) The human CENP-A centromeric nucleosome-associated complex. Nature Cell Biology, 8, 458-69



Lars´ pre-PhD Papers

Leiden Institute for Chemistry, Leiden University


Masakazu Kobayashi, Francis Figaroa, Nico Meeuwenoord, Lars E.T. Jansen and Gregg Siegal (2006) Characterization of the DNA binding and structural properties of the BRCT region of human replication factor C p140 subunit. J Biol Chem., 281, 4308-17


Robert C. A. M. van Waardenburg, Laurina A. de Jong, Maria A. J. van Eijndhoven, Caroline Verseyden, Dick Pluim, Lars E.T. Jansen, Mary-Ann Bjornsti, and Jan H. M. Schellens (2004) Platinated DNA Adducts Enhance Poisoning of DNA Topoisomerase I by Camptothecin. J. Biol. Chem., 279, 54502-9.


Lars E.T. Jansen, Ana I. Belo, Rinske Hulsker and Jaap Brouwer (2002) Transcription elongation factor Spt4 mediates loss of phosphorylated RNA polymerase II transcription in response to DNA damage. Nucleic Acids Res., 30, 3532-9.



Elies C. Woudstra, Chris Gilbert, Jane Fellows, Lars E.T. Jansen, Jaap Brouwer, Hediye Erdjument-Bromage, Paul Tempst and Jesper Q. Svejstrup (2002) A Rad26-Def1 complex co-ordinates repair and RNA polymerase II proteolysis in response to DNA damage. Nature. 415, 929-33.


Lars E.T. Jansen, Hans den Dulk, Rosalba M. Brouns, Martina de Ruijter, Jourica A. Brandsma and Jaap Brouwer (2000) Spt4 modulates Rad26 requirement in transcription-coupled nucleotide excision repair. EMBO J., 19, 6498-507.



Lars E.T. Jansen, Richard A. Verhage and Jaap Brouwer (1998) Preferential binding of yeast Rad4-Rad23 complex to damaged DNA. J Biol Chem., 273, 33111-4.




Lars´ even earlier Papers

Institute for Molecular Plant sciences, Leiden University


Annette C. Vergunst, Lars E.T. Jansen, Paul F. Fransz, J. Hans de Jong and Paul J.J. Hooykaas (2000) Cre/lox-mediated recombination in Arabidopsis: evidence for transmission of a translocation and a deletion event. Chromosoma. 109, 287-97.



Annette C. Vergunst, Lars E.T. Jansen and Paul J. Hooykaas (1998) Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase. Nucleic Acids Res., 26, 2729-34.



Links

Our departmental web page at Biochemistry, University of Oxford
Lars is the William R Miller Fellow and Tutor in Biochemistry at St Edmund Hall



Our Science in basic terms. Lars wrote a lay blog on genetics and epigenetics for Teddy Hall, Oxford

In the spotlight

Recently ...

How do cells remeber the past?
In our latest preprint we report the discovery of a unique set of histone modifications that mark "primed" chromatin, implicated in maintaining a heritable memory of exposure to the cytokine interferon-gamma. Previously we discovered that the transcription factor STAT1 induces a mitotically stable memory of exposure to interferon. This primes cells to future encounters with the cytokine. See our recent paper on this fascinating phenomenon. Destruction of a centromere
We discovered that centromeric chromatin, while featuring stably inherited CENP-A nucleosomes, can be rapidly destabilized by regulated and SUMO-targeted recruitment of the p97 segregase. This offers new insight into how human centromeres are dynamically maintained. Check out our paper !

Birth of a neocentromere
We developed a system to isolate a spontaneously formed neocentromere in the context of the complex human genome. Check out our paper on this fascinating piece of biology!

Our first paper on mechanisms of long term transcriptional memory in Molecular Cell. Wojek Siwek and Sahar Tehrani identify a set of clustered human genes that display long-term priming following activation by interferon-g.
We discovered that local cohesin binding contributes to restricting memory.

The first description of centromeres in human induced or natural pluripotent stem cells! Inês Milagre demonstrates centromeres in this key cell type have a reduced size and limited pool of CENP-B and CENP-A chromatin.

What does the international space station have in common with human centromeres?

Everything we know and don´t know about centromere inheritance and CENP-A nucleosome stability is now wonderfully summarized and digested by Sreyoshi Mitra with help from Bharath Srinivasan in her review in the Journal of Cell Biology

Our Marina Murillo wrote a comprehensive viewpoint on how centromere research developed over the last four decades. She explains how neocentromeres taught us about both genetic and chromatin-based elements driving centromere formation and inheritance. Read it here!

Check out our paper in Nature Comm. We identified a series of new proteins required to stably transmit CENP-A chromatin through cell division. Most critically, we uncover a SUMOylation cycle that is essential to maintain an intact centromere complex.

In Nature Ecology & Evolution we report how chromatin-based heritable gene silencing in a yeast population can accelerate adaption to a novel environment. In a dramatic departure from human centromeres and mammalian transcription we explored this key question in evolution in a wonderful collaboration with Lilia Perfeito @ Gulbenkian, Portugal.